Trypan Blue solution

Trypan blue is a dye used to distinguish between live and dead cells. It is a vital stain that is not absorbed by healthy viable cells, but stains cells with a damaged cell membrane.[2] This way, only dead cells can be counted. The method is sometimes referred to as the dye exclusion method.


detachment enzyme

Sigma Aldrich

sterility sterile-filtered
form liquid
storage condition dry at room temperature
concentration 0,40%
shipped in ambient
T8154 Trypan Blue solution
0.4%, liquid, sterile-filtered, suitable for cell culture
BR717810 BRAND® counting chamber BLAUBRAND® Neubauer improved
without clips, bright-line


The ruled surface is 0.1mm below the cover glass, limiting the volume of blood or fluid over a square mm at and over each of 400 squares (within the central square mm) to 0.00025 cu. mm. Contact of the flat, polished cover glass surfaces with cover glass supports produces an exact volume of fluid over the counting area.

The difference in surface tension characteristics between the metallic surface on the chamber and the polished cover glass assures smooth capillarity for precise loading and more even cell distribution.