Thermo Scientific™ Multiskan Sky Microplate Spectrophotometer

The Multiskan Sky Microplate Spectrophotometer is exceptionally convenient and easy to use for virtually any photometric research application, especially nucleic acid and protein analyses.

It is ideal for multi-user environments where a variety of endpoint, kinetic, and
spectral assays are run. Results are provided quickly and presented conveniently on Multiskan Sky Microplate Spectrophotometer with touch screen.

In addition, Multiskan Sky offer multiple connectivity options including unique access to Thermo Fisher Connect cloud-based tools. Additionally, the intuitive Thermo ™ SkanIt™ PC Software is powerful enough to address even the most challenging applications.

Details

Category:
Microplate Reader

Manufacture:
Thermo Scientific

Benefit

Stand-alone, can be operated directly on the instrument
Self-diagnostic feature
Free wavelength selection
Compatible with 96- and 384-well plates, with and without lids, or cuvette
Fast and accurate measurements
Integrated shaker and incubator
Automation compatibility for integration with SkanIt Software

Specification

Wavelength selection

Monochromator

Light source

Xenon flash lamp

Wavelength range

200–1000 nm

Read-out range

0-3 Abs

Bandwidth

<2.5 nm

Resolution

0.001 Abs

Linearity @450 nm

0–2.5 Abs, ± 2%

Accuracy @450 nm

± 1% (0–3 Abs) or ± 0.003 Abs

± 2% (2.0–2.5 Abs)

Precision @450 nm

SD <0.003 Abs or CV< 1.0%

Plate type

96- and 384-well plates

Cuvette

Dimensions (H x W x D): 40-58 x 12.5 x 12.5 mm

Beam center height: 8.5 mm, Beam window: ≥2 mm

Low-volume µDrop Plate

Measurement area : 16 measurement positions in a 2 x 8 matrix

Sample Volume : 2µL to 10µL

Plate Shaking

Linear

Measurement Speed (from A1 back to A1)

6s, 96-well plate

10s, 384-well plate

Spectral Scanning Speed

10 s from 200 to 1000 nm with 1 nm steps

Incubation

from ambient +4 ^oC t0 45 ^oC

Application

Nucleic acids (DNA, RNA) quantitation

Protein assays (direct quantitation and colorimetric assays)

Cell proliferation, viability, and cytotoxicity assays

Apoptosis assays

Enzymatic activity assays

Spectral and kinetic assays

Immunoassays (ELISA)

Endotoxin assays

Bacterial growth assays

Catalog no.

Catalog no.	Description
51119700DP	Multiskan Sky + Cuvette Port + µDrop Plate
51119600	Multiskan Sky

Resource

Brochure

Application notes

Orthogonal approaches for studying bacterial biofilms using photometric detection with a Multiskan Sky Microplate Spectrophotometer and brightfield imaging with an EVOS FL Auto Imaging System
Simplified workflow for Instant ELISA kits using the Multiskan Sky Microplate Spectrophotometer and Wellwash Versa Microplate Washer
A theoretical and practical guide for spectrophotometric determination of protein concentrations at 280 nm
Photometric DNA quantification at 260 nm using the Multiskan Sky Microplate Spectrophotometer user interface
Photometric DNA quantification using the Multiskan Sky Microplate Spectrophotometer operated with SkanIt Software

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