Thermo Scientific™ Multiskan Sky Microplate Spectrophotometer

The Multiskan Sky Microplate Spectrophotometer is exceptionally convenient and easy to use for virtually any photometric research application, especially nucleic acid and protein analyses.

It is ideal for multi-user environments where a variety of endpoint, kinetic, and
spectral assays are run. Results are provided quickly and presented conveniently on Multiskan Sky Microplate Spectrophotometer with touch screen.

In addition, Multiskan Sky offer multiple connectivity options including unique access to Thermo Fisher Connect cloud-based tools. Additionally, the intuitive Thermo ™ SkanIt™ PC Software is powerful enough to address even the most challenging applications.


Microplate Reader

Thermo Scientific

  • Stand-alone, can be operated directly on the instrument
  • Self-diagnostic feature
  • Free wavelength selection
  • Compatible with 96- and 384-well plates, with and without lids, or cuvette
  • Fast and accurate measurements
  • Integrated shaker and incubator
  • Automation compatibility for integration with SkanIt Software
Wavelength selection : Monochromator
Light source : Xenon flash lamp
Wavelength range : 200–1000 nm
Read-out range : 0-3 Abs
Bandwidth : <2.5 nm
Resolution : 0.001 Abs
Linearity @450 nm : 0–2.5 Abs, ± 2%
Accuracy @450 nm : ± 1% (0–3 Abs) or ± 0.003 Abs

± 2% (2.0–2.5 Abs)

Precision @450 nm : SD <0.003 Abs or CV< 1.0%
Plate type :
96- and 384-well plates
Cuvette : Dimensions (H x W x D): 40-58 x 12.5 x 12.5 mm

Beam center height: 8.5 mm, Beam window: ≥2 mm

Low-volume µDrop Plate : Measurement area : 16 measurement positions in a 2 x 8 matrix

Sample Volume : 2µL to 10µL

Plate Shaking : Linear
Measurement Speed (from A1 back to A1) : 6s, 96-well plate

10s, 384-well plate

Spectral Scanning Speed : 10 s from 200 to 1000 nm with 1 nm steps
Incubation : from ambient +4 oC t0 45 oC
Application : Nucleic acids (DNA, RNA) quantitation

Protein assays (direct quantitation and colorimetric assays)

Cell proliferation, viability, and cytotoxicity assays

Apoptosis assays

Enzymatic activity assays

Spectral and kinetic assays

Immunoassays (ELISA)

Endotoxin assays

Bacterial growth assays

Catalog no. Description
51119700DP Multiskan Sky + Cuvette Port + µDrop Plate
51119600 Multiskan Sky


  • Orthogonal approaches for studying bacterial biofilms using photometric detection with a Multiskan Sky Microplate Spectrophotometer and brightfield imaging with an EVOS FL Auto Imaging System
  • Simplified workflow for Instant ELISA kits using the Multiskan Sky Microplate Spectrophotometer and Wellwash Versa Microplate Washer
  • A theoretical and practical guide for spectrophotometric determination of protein concentrations at 280 nm
  • Photometric DNA quantification at 260 nm using the Multiskan Sky Microplate Spectrophotometer user interface
  • Photometric DNA quantification using the Multiskan Sky Microplate Spectrophotometer operated with SkanIt Software