RPMI-1640 Medium

RPMI 1640 Medium was developed at Roswell Park Memorial Institute
in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.



Sigma Aldrich

Appearance (Turbidity) Clear
Appearance (Form) Solution
pH 7.0 – 7.6
Osmolality 264 – 292 mOs/kg
Cell Culture Testing – MTT Pass
Cell Line Cell Line – Cell Types
Sterility Pass
Endotoxin Level <= 1 EU/ml
Glucose Concentration 1.8 – 2.2 g/l
R8758 RPMI-1640 Medium
With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture


cell culture | mammalian: suitable