M&S Basal Medium contains macronutrients that
include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).
Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog medium (M9274) contains the micronutrients and vitamins of the original classic formulation plus sucrose and agar. It can be supplemented with auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.