Dulbecco’s Modified Eagle’s Medium

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium
Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells.



Sigma Aldrich

Appearance (Turbidity) Clear
Appearance (Form) Solution
pH 7.0 – 7.6
Osmolality 326 – 360 mOs/kg
Cell Culture Testing – MTT Pass
Cell Line Cell Line – Cell Types
Sterility Pass
Endotoxin Level <= 1 EU/ml
Glucose Concentration 4.05 – 4.95 g/l
D6429 Dulbecco’s Modified Eagle’s Medium – high glucose
With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
D5546 Dulbecco’s Modified Eagle’s Medium – low glucose
With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture


cell culture | mammalian: suitable